The SMTP server requires a secure connection or the client was not authenticated?

MailMessage Msg = new MailMessage();
           SmtpClient Smtp = new SmtpClient();
           EmailID = txtEmailID.Text.Trim();
           Msg.From = new MailAddress("PrasadAnumolu999@gmail.com");
           Msg.To.Add(EmailID);
           Msg.Subject = "Please Confirm You Activation Link";
           ActivationURL = Server.HtmlEncode("http://localhost:2759/EmailVarification.aspx" + "&EmailID=" + EmailID);
           Msg.Body="Hi" +txtEmailID.Text.Trim() +"!\n" +
               "Thanks For You Interest For Visiting My Web Site <a href='http://stuffuser.blogspot.in/'> STUFFUSER.in</a>" +
                        " Please <a href='" + ActivationURL + "'>click here to activate</a>  your account and enjoy our services. \nThanks!";

           Msg.IsBodyHtml = true;
           Smtp.Port = 25;
           Smtp.Host = "Smtp.Gmail.com";
           Smtp.EnableSsl = true;
           Smtp.Credentials = new NetworkCredential("PrasadAnumolu@gmail.com", "sai!#%&(99");
           Smtp.Send(Msg);