problem with webservice

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C#

C#4.0

I have a problem with web service.

I have created a web service using asp.net 2010, when i run by pressing F5 everything is fine. It'll worked properly.
But when i publish it using IIS, after click on "invoke" it shows me error

System.Data.SqlClient.SqlException: Login failed for user 'FINGERTIPS3\ASPNET'.
   at System.Data.SqlClient.SqlInternalConnection.OnError(SqlException exception, Boolean breakConnection)
   at System.Data.SqlClient.TdsParser.ThrowExceptionAndWarning()
   at System.Data.SqlClient.TdsParser.Run(RunBehavior runBehavior, SqlCommand cmdHandler, SqlDataReader dataStream, BulkCopySimpleResultSet bulkCopyHandler, TdsParserStateObject stateObj)
   at System.Data.SqlClient.SqlInternalConnectionTds.CompleteLogin(Boolean enlistOK)
   at System.Data.SqlClient.SqlInternalConnectionTds.AttemptOneLogin(ServerInfo serverInfo, String newPassword, Boolean ignoreSniOpenTimeout, TimeoutTimer timeout, SqlConnection owningObject)
   at System.Data.SqlClient.SqlInternalConnectionTds.LoginNoFailover(ServerInfo serverInfo, String newPassword, Boolean redirectedUserInstance, SqlConnection owningObject, SqlConnectionString connectionOptions, TimeoutTimer timeout)
   at System.Data.SqlClient.SqlInternalConnectionTds.OpenLoginEnlist(SqlConnection owningObject, TimeoutTimer timeout, SqlConnectionString connectionOptions, String newPassword, Boolean redirectedUserInstance)
   at System.Data.SqlClient.SqlInternalConnectionTds..ctor(DbConnectionPoolIdentity identity, SqlConnectionString connectionOptions, Object providerInfo, String newPassword, SqlConnection owningObject, Boolean redirectedUserInstance)
   at System.Data.SqlClient.SqlConnectionFactory.CreateConnection(DbConnectionOptions options, Object poolGroupProviderInfo, DbConnectionPool pool, DbConnection owningConnection)
   at System.Data.ProviderBase.DbConnectionFactory.CreatePooledConnection(DbConnection owningConnection, DbConnectionPool pool, DbConnectionOptions options)
   at System.Data.ProviderBase.DbConnectionPool.CreateObject(DbConnection owningObject)
   at System.Data.ProviderBase.DbConnectionPool.UserCreateRequest(DbConnection owningObject)
   at System.Data.ProviderBase.DbConnectionPool.GetConnection(DbConnection owningObject)
   at System.Data.ProviderBase.DbConnectionFactory.GetConnection(DbConnection owningConnection)
   at System.Data.ProviderBase.DbConnectionClosed.OpenConnection(DbConnection outerConnection, DbConnectionFactory connectionFactory)
   at System.Data.SqlClient.SqlConnection.Open()
   at WebTest.Service1.HelloWorld() in D:\Gaurav\Task18-02-2013\WebserviceTest\WebTest\WebTest\Service1.asmx.cs:line 30.

How i solve this error, Please help me as soon as possible..
Thanx in advanced..