Access to the path \mediatoolkit is denied

ConvertFile("E:\kunden\homepages\19\d664110395\www\example\catalog\videos\" & imageFilename, "E:\kunden\homepages\19\d664110395\www\example\catalog\videos\new\" & imageFilename, Options.VideoSize.Hd720)

    Private Function ConvertFile(ByVal sourceFile As String, ByVal destinationFile As String,
                             ByVal videoSize As MediaToolkit.Options.VideoSize) As MediaFile

    Dim inputFile = New MediaFile With {.Filename = sourceFile}
    Dim outputFile = New MediaFile With {.Filename = destinationFile}

    Using engine = New Engine()
        'engine.ConvertProgressEvent = RaiseEvent engine_ConvertProgressEvent
        'engine.ConversionCompleteEvent = RaiseEvent engine_ConversionCompleteEvent
        engine.Convert(inputFile, outputFile, New MediaToolkit.Options.ConversionOptions With {
        .VideoSize = videoSize
    })
        engine.GetMetadata(inputFile)
        engine.GetMetadata(outputFile)
    End Using

    Dim inputMeta As Metadata = inputFile.Metadata
    Dim outputMeta As Metadata = outputFile.Metadata

    Return outputFile
End Function