First of all, you must include all the images into your project, prefreably into images folder. Then use Server.MapPath to find our the image existance. Server.MapPath return the physical path of the image file so that you can use it to verify the existance.
string imgpath = Server.MapPath("~\images\yourimg.jpg");
if(System.IO.File.Exists(imgpath))
...
else
...