Getting 500 internal server error when executing move_uploaded_files

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PHP

I am getting 500 internal Server Error here when uploading a file to the database inside the website image path folder.

In localhost same code is working but if I run the same application on Live site, i am getting 500 error.

*Checked permission of directory, it's 777

Please help me out with this problem.

Thanks in advance!

What I have tried:

$uploadfile = 'manuscript/';

$file_name = $_FILES['mdoc']['name'];
$file_size =$_FILES['mdoc']['size'];
$file_tmp =$_FILES['mdoc']['tmp_name'];
$file_type=$_FILES['mdoc']['type'];
$x=explode('.',$file_name);
$file_name=$id.'-'.$x[0].'.'.$x[1];
//$moved = move_uploaded_file($file_tmp, $uploadfile.$file_name);
if (move_uploaded_file($_FILES['mdoc']['tmp_name'], $uploadfile)) {
echo "File is valid, and was successfully uploaded.\n";
} else {
echo "Upload failed";
}