removing white from a string

mystring.Replace(" /","/");

Use Below code for your problem.. it will definitely solve your problem.

string str = @"http://localhost:23766/WebSite2/NewFolder1/Division of ICT/Dept of MIS and Operations/Software Request Form/Samabula /HR Policy Review for HRC_RC_3052012_final.pdf";
string[] strsplit = str.Split('/');
str =null;
foreach (string item in strsplit) { str = str + item.Trim() + "/"; }

static void Main(string[] args)
       {
            StringToTrim();
            Console.ReadLine();
       }
       private static void StringToTrim()
       {
           String str = "My Name Is Niranjan Kumar";
           string delimeter = " ";
           String[] Result = str.Split(new[] { delimeter }, StringSplitOptions.None);
           for (int i = 0; i < Result.Length; i++)
           {
               Console.Write(Result[i]);
           }
       }

 public static int Main(string[] args)
    {
        Console.WriteLine(URL.PrepareURL("http://localhost:23766/WebSite2/NewFolder1/Division of ICT/Dept of MIS and Operations/Software Request Form/Samabula /HR Policy Review for HRC_RC_3052012_final.pdf"));
        Console.ReadLine();
        return 0;
    }
}
public static class URL
{
    static readonly Regex feet = new Regex(@"([0-9]\s?)'([^'])", RegexOptions.Compiled);
    static readonly Regex inch1 = new Regex(@"([0-9]\s?)''", RegexOptions.Compiled);
    static readonly Regex inch2 = new Regex(@"([0-9]\s?)""", RegexOptions.Compiled);
    static readonly Regex num = new Regex(@"#([0-9]+)", RegexOptions.Compiled);
    static readonly Regex dollar = new Regex(@"[$]([0-9]+)", RegexOptions.Compiled);
    static readonly Regex percent = new Regex(@"([0-9]+)%", RegexOptions.Compiled);
    static readonly Regex sep = new Regex(@"[\s_/\\+:.]", RegexOptions.Compiled);
    static readonly Regex empty = new Regex(@"[^-A-Za-z0-9]", RegexOptions.Compiled);
    static readonly Regex extra = new Regex(@"[-]+", RegexOptions.Compiled);

    public static string PrepareURL(string str)
    {
        str = str.Trim().ToLower();
        str = str.Replace("&", "and");

        str = feet.Replace(str, "$1-ft-");
        str = inch1.Replace(str, "$1-in-");
        str = inch2.Replace(str, "$1-in-");
        str = num.Replace(str, "num-$1");

        str = dollar.Replace(str, "$1-dollar-");
        str = percent.Replace(str, "$1-percent-");

        str = sep.Replace(str, "-");

        str = empty.Replace(str, string.Empty);
        str = extra.Replace(str, "-");

        str = str.Trim('-');
        return str;
    }
}

protected void Button1_Click(object sender, EventArgs e)
    {
        string str = "http://localhost:23766/WebSite2/NewFolder1/Division of ICT/Dept of MIS and Operations/Software Request Form/Samabula /HR Policy Review for HRC_RC_3052012_final.pdf";
        string[] str1 = str.Split(' ');
        Label1.Text = "";
        for (int i = 0; i < str1.Length; i++)
        {
            Label1.Text = Label1.Text + str1[i];
        }

    }