you can get the all the files in image folder as below
string[] filesindirectory = Directory.GetFiles(Server.MapPath("~/Image"));
then create loop and add images to excel sheet
Table table = new Table();
foreach(string img in filesindirectory)
{
System.Web.UI.WebControls.Image TEST_IMAGE = new System.Web.UI.WebControls.Image();
TEST_IMAGE.ImageUrl = "Image/"+ Path.GetFileName(img) ;
TEST_IMAGE.ImageUrl = this.GetAbsoluteUrl(TEST_IMAGE.ImageUrl);
TableRow row = new TableRow();
row.Cells.Add(new TableCell());
row.Cells[0].Controls.Add(TEST_IMAGE);
table.Rows.Add(row);
}