Here is one sample working code snippet. You have to provide Relative paths with ImageURL.
protected void Button1_Click(object sender, EventArgs e)
{
if (FileUpload1.HasFile)
{
string filename = Guid.NewGuid().ToString() + ".jpg";
FileUpload1.PostedFile.SaveAs(Server.MapPath("Images\\") +filename);
aspimage.ImageUrl = "~/Images/" + filename;
}
}