Here is the sample code to bind the image from the folder as:
<asp:DataList ID="dtlist" runat="server" RepeatColumns="4" CellPadding="5">
<ItemTemplate>
<asp:Image Width="100" ID="Image1" ImageUrl='<%# Bind("Name", "~/Images/{0}") %>' runat="server" />
<itemstyle bordercolor="Brown" borderstyle="dotted" borderwidth="3px" horizontalalign="Center">
VerticalAlign="Bottom" />
</itemstyle>
Code behind to load the image as:
protected void BindDataList()
{
DirectoryInfo dir = new DirectoryInfo(MapPath("Images"));
FileInfo[] files = dir.GetFiles();
ArrayList listItems = new ArrayList();
foreach (FileInfo info in files)
{
listItems.Add(info);
}
dtlist.DataSource = listItems;
dtlist.DataBind();
}