How do I get around the access denied problem when uploading an image from a web app.

connection.Open();
string filePath =
Path.GetFileName(oFile.PostedFile.FileName.ToString());
string uploadPath = Server.MapPath("~/upload");
oFile.PostedFile.SaveAs(Path.Combine(uploadPath,
oFile.PostedFile.FileName));

string sql = "INSERT INTO `database`.`table` (image) VALUES (@file)";
using (var cmd = new MySqlCommand(sql, connection))
{
    cmd.Parameters.AddWithValue("@file", File.ReadAllBytes(uploadPath
     + "\\" + filePath));
    cmd.ExecuteNonQuery();
}
connection.Close();

<pre lang="HTML">Access to the path 'E:\www\website\website\wwwroot\upload\Image-01.jpg' is 
denied.
Description: An unhandled exception occurred during the execution of the 
current web request. Please review the stack trace for more information 
about the error and where it originated in the code. 

 Exception Details: System.UnauthorizedAccessException: Access to the path 
 'E:\www\website\website\wwwroot\upload\Image-01.jpg' 
 is denied. 

ASP.NET is not authorized to access the requested resource. Consider 
granting access rights to the resource to the ASP.NET request identity. 
ASP.NET has a base process identity (typically {MACHINE}\ASPNET on IIS 5 or 
Network Service on IIS 6 and IIS 7, and the configured application pool 
identity on IIS 7.5) that is used if the application is not impersonating. 
If the application is impersonating via , the 
identity will be the anonymous user (typically IUSR_MACHINENAME) or the 
authenticated request user. 

To grant ASP.NET access to a file, right-click the file in File Explorer, 
choose "Properties" and select the Security tab. Click "Add" to add the 
appropriate user or group. Highlight the ASP.NET account, and check the 
boxes for the desired access.

Source Error: 

An unhandled exception was generated during the execution of the current web 
request. Information regarding the origin and location of the exception can 
be identified using the exception stack trace below.


Stack Trace: 
[UnauthorizedAccessException: Access to the path 
'E:\www\premileh\website\wwwroot\upload\Image-01.jpg' 
is denied.]
System.IO.__Error.WinIOError(Int32 errorCode, String maybeFullPath) +417
System.IO.FileStream.Init(String path, FileMode mode, FileAccess access, 
Int32 rights, Boolean useRights, FileShare share, Int32 bufferSize, 
FileOptions options, SECURITY_ATTRIBUTES secAttrs, String msgPath, Boolean 
bFromProxy, Boolean useLongPath, Boolean checkHost) +1217
System.IO.FileStream..ctor(String path, FileMode mode, FileAccess access, 
FileShare share, Int32 bufferSize, FileOptions options, String msgPath, 
Boolean bFromProxy) +130
System.IO.FileStream..ctor(String path, FileMode mode) +97
System.Web.HttpPostedFile.SaveAs(String filename) +96
application.ItemUploadPage.ImageUploader()in
C:\Users\folder\folder\folder\folder\folder\folder\folder\folder.aspx.cs:133
app.apppage.UploadBtn_Click(Object sender, EventArgs e) 
\Users\folder\folder\folder\folder\folder\folder\app
\ItemUploadPage.aspx.cs:97
System.Web.UI.WebControls.Button.OnClick(EventArgs e) +11758848
System.Web.UI.WebControls.Button.RaisePostBackEvent(String 
eventArgument)+150
System.Web.UI.Page.ProcessRequestMain(Boolean 
includeStagesBeforeAsyncPoint,Boolean includeStagesAfterAsyncPoint) +5028